The biosynthesis of isoflavonoids involves several enzymes; These are:
, also known as Isoflavonoid synthase, is an enzyme that uses liquiritigenin (a flavanone), O<sub>2</sub>, NADPH and H<sub>+</sub> to produce 2,7,4'-trihydroxyisoflavanone (an isoflavonoid), H<sub>2</sub>O and NADP<sup>+</sup>.