In enzymology, glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are D-glyceraldehyde 3-phosphate, phosphate (P<sub>i</sub>), and oxidised nicotinamide adenine dinucleotide (NAD<sup>+</sup>). Its products are 1,3-bisphosphoglyceric acid, reduced NADH, and a proton. The enzyme can use nicotinamide adenine dinucleotide phosphate as an alternative cofactor.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of a donor with NAD+ or NADP+ as an acceptor. The systematic name of this enzyme class is D-glyceraldehyde 3-phosphate:NAD(P)+ oxidoreductase (phosphorylating). Other names in common use include triosephosphate dehydrogenase (NAD(P)) (phosphorylating), and glyceraldehyde-3-phosphate dehydrogenase (NAD(P)) (phosphorylating).
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .