The Shire of Kyneton was a local government area about northwest of Melbourne, the state capital of Victoria, Australia. The shire covered an area of , and existed from 1859 until 1995.
Kyneton was incorporated as a road district on 8 February 1859, and became a shire on 18 January 1865. Martin McKenna, a brewer, miner, pastoralist and politician, was the first president of the Shire of Kyneton.
On 5 February 1913 and 9 October 1921, it annexed parts of the Shires of Bacchus Marsh and Ballan respectively, while on 1 October 1915, it absorbed the Borough of Malmsbury, which had been created on 19 October 1861 with an area of .
On 19 January 1995, the Shire of Kyneton was abolished, and along with the Shires of Gisborne, Newham and Woodend and Romsey, was merged into the newly created Shire of Macedon Ranges. A small part of the shire was also transferred to the newly created Hepburn Shire.
The Shire of Kyneton was divided into four ridings, each of which elected three councillors:
<small>* Council seat.</small>
<small>* Estimate in the 1958 Victorian Year Book.</small>