is a rural district located in southeastern Yamanashi Prefecture, Japan.
As of 1 October 2020, the district had an estimated population of 48,970 with a density of 116,3 persons per km<sup>2</sup>. The total area was 421 km<sup>2</sup>.
The districts consists of two towns and four villages:
The district formerly included the cities of Fujiyoshida, Tsuru and a portion of the city of Uenohara.
Minamitsuru District was founded during the early Meiji period establishment of the municipalities system on July 22, 1878 and initially consisted of 21 villages.