Mathura Assembly constituency is one of the 403 constituencies of the Uttar Pradesh Legislative Assembly, India. It is a part of the Mathura district and one of the five assembly constituencies in the Mathura Lok Sabha constituency.
The first election in this assembly constituency was held in 1957 after the delimitation order was passed in 1956. After the "Delimitation of Parliamentary and Assembly Constituencies Order" was passed in 2008, the constituency was assigned identification number 84.
Extent of Mathura Assembly constituency is PCs Jaisinghpura, Ganesara, Naugaon, Maholi, Narholi, Mathura Bangar, Aurangabad, Dhangaon, Aduki of Mathura KC, PCs 1Sakraya Bangar, Vrindaban of Vrindaban KC, Chhatikara, Jait, Vrindaban MB, Mathura MB & Mathura (CB) of Mathura Tehsil.