Mathura Lok Sabha constituency () is one of the 80 Lok Sabha (parliamentary) constituencies in the Indian state of Uttar Pradesh. This constituency covers the entire Mathura district. According to Election Commission of India 2009 reports, the Mathura Parliamentary constituency (constituency number 17) has a total of 1,341,649 electorates out of which 593,726 are females and 747,923 are males.
Presently, Mathura Lok Sabha constituency comprises five Vidhan Sabha (Uttar Pradesh legislative assembly) segments.