Lottieville is an unincorporated community in Gilchrist County, Florida, United States. It is located on State Road 26, approximately west of Trenton.
Lottieville is located at , its elevation .
Lottieville was a stop on the Atlantic Coast Line Railroad JacksonvilleâÂÂWilcox Line, between the communities of Wilcox and Trenton. Today, the line is used by the Nature Coast State Trail.