Khlong Khoi (, ) is one of the twelve subdistricts (tambon) of Pak Kret District, in Nonthaburi Province, Thailand. Neighbouring subdistricts are (from north clockwise) Lat Lum Kaeo, Khlong Phra Udom (Pathum Thani Province), Bang Khu Wat, Bang Tanai, Khlong Phra Udom (Nonthaburi Province), Bang Phlap, Lahan, Lam Pho, Lahan and Namai. In 2020 it had a total population of 8,383 people.
The subdistrict is subdivided into 12 administrative villages (muban).
The whole area of the subdistrict is covered by Khlong Khoi Subdistrict Administrative Organization ().