Acquanegra Cremonese (Cremunés: ) is a comune (municipality) in the Province of Cremona in the Italian region Lombardy, located about southeast of Milan and about northwest of Cremona.
Acquanegra Cremonese borders the following municipalities: Crotta d'Adda, Grumello Cremonese ed Uniti, Sesto ed Uniti, Spinadesco.