The 2016 Copa Libertadores second stage was played from 16 February to 21 April 2016. A total of 32 teams competed in the second stage to decide the 16 places in the final stages of the 2016 Copa Libertadores.
The draw of the tournament was held on 22 December 2015, 20:30 PYST (), at the CONMEBOL Convention Centre in Luque, Paraguay.
Starting from this season, teams were seeded by the newly established CONMEBOL ranking of the Copa Libertadores (except for teams from Mexico which were not ranked and thus seeded last in all draws), taking into account of the following three factors:
<onlyinclude></onlyinclude> The six winners of the first stage which joined the 26 direct entrants were as follows:
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The fixture list was determined by the draw as follows:
The matches were played on 16âÂÂ18, 23âÂÂ25 February, 1âÂÂ3, 8âÂÂ10, 15âÂÂ17 March, 5âÂÂ7, 12âÂÂ14 and 19âÂÂ21 April 2016.
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