In enzymology, xylitol oxidase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are xylitol and oxygen. Its products are D-xylose and hydrogen peroxide. Other sugar alcohols, including sorbitol, can be oxidised by this enzyme.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is xylitol:oxygen oxidoreductase.