In enzymology, vellosimine dehydrogenase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are 10-deoxysarpagine and oxidised nicotinamide adenine dinucleotide phosphate (NADP<sup>+</sup>). Its products are the indole alkaloid vellosimine, reduced NADPH, and a proton.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD<sup>+</sup> or NADP<sup>+</sup> as acceptor. The systematic name of this enzyme class is 10-deoxysarpagine:NADP<sup>+</sup> oxidoreductase. This enzyme participates in indole and ipecac alkaloid biosynthesis.