In enzymology, a vanillyl-alcohol oxidase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are vanillyl alcohol and O<sub>2</sub>, whereas its two products are vanillin and H<sub>2</sub>O<sub>2</sub>.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is vanillyl alcohol:oxygen oxidoreductase. This enzyme is also called 4-hydroxy-2-methoxybenzyl alcohol oxidase. This enzyme participates in 2,4-dichlorobenzoate degradation. It employs one cofactor, FAD.
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes , , and .