In enzymology, an ureidoglycolate hydrolase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are (S)-ureidoglycolate and H<sub>2</sub>O, whereas its 3 products are glyoxylate, NH<sub>3</sub>, and CO<sub>2</sub>.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is (S)-ureidoglycolate amidohydrolase (decarboxylating). This enzyme participates in purine metabolism.
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes , , , and .