In enzymology, a thiophene-2-carbonyl-CoA monooxygenase () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are thiophene-2-carbonyl-CoA, an electron acceptor AH<sub>2</sub>, and O<sub>2</sub>. Its three products are 5-hydroxythiophene-2-carbonyl-CoA, the reduction product A, and H<sub>2</sub>O.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O<sub>2</sub> as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O miscellaneous. The systematic name of this enzyme class is thiophene-2-carbonyl-CoA, hydrogen-donor:oxygen oxidoreductase. Other names in common use include thiophene-2-carboxyl-CoA dehydrogenase, thiophene-2-carboxyl-CoA hydroxylase, and thiophene-2-carboxyl-CoA monooxygenase.