A thiocyanate hydrolase () is an enzyme belonging to the family of hydrolases. The systematic name of this enzyme class is thiocyanate aminohydrolase. This enzyme catalyzes the chemical reaction:
The mechanism is proposed to involve a metal thiocyanate complex.
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes , , , and .
A second thiocyanate hydrolase with copper at its active site catalyzes its conversion to cyanate: