tRNA pseudouridine<sup>32</sup> synthase (, RluA, pseudouridine synthase RluA, Pus9p, Rib2/Pus8p) is an enzyme with systematic name tRNA-uridine<sup>32</sup> uracil mutase. This enzyme catalyses the following chemical reaction
The dual enzyme from Escherichia coli also catalyses the formation of pseudouridine<sup>746</sup> in 23S rRNA.