TRNA (adenine<sup>58</sup>-N<sup>1</sup>)-methyltransferase (, tRNA m1A58 methyltransferase, tRNA (m1A58) methyltransferase, TrmI, tRNA (m1A58) Mtase, Rv2118cp, Gcd10p-Gcd14p, Trm61p-Trm6p) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (adenine<sup>58</sup>-N<sup>1</sup>)-methyltransferase. This enzyme catalyses the following chemical reaction
The enzyme specifically methylates adenine<sup>58</sup> in tRNA.