In enzymology, a sarsasapogenin 3ò-glucosyltransferase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are UDP-glucose and sarsasapogenin [(3ò,5ò,25S)-spirostan-3-ol], whereas its two products are UDP and sarsasapogenin 3-O-ò-<small>D</small>-glucoside.
The enzyme was first isolated from the root of the common asparagus (Asparagus officinalis). It is specific for substrate sterols with the uncommon 5ò-configuration (sarsasapogenin and smilagenin), that is with a cis-linkage between the A and B rings of the steroid nucleus.
This enzyme belongs to the family of glycosyltransferases, specifically the hexosyltransferases. The systematic name of this enzyme class is UDP-glucose:(3ò,5ò,25S)-spirostan-3-ol 3-O-ò-<small>D</small>-glucosyltransferase. This enzyme is also called uridine diphosphoglucose-sarsasapogenin glucosyltransferase.