Ruberlysin (, Crotalus ruber metalloendopeptidase II, hemorrhagic toxin II) is an enzyme. This enzyme catalyses the following chemical reaction
Cleavage of His<sup>10</sup>-Leu, Ala<sup>14</sup>-Leu, Tyr<sup>16</sup>-Leu and Gly<sup>23</sup>-Phe bonds in the B chain of insulin; His-Pro, Pro-Phe, and Trp-Ser of angiotensin I; and Gly-Phe of Met enkephalin
This endopeptidase is present in the venom of the red rattlesnake (Crotalus ruber ruber).
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