Pyrogallol 1,2-oxygenase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are pyrogallol and oxygen. Its product is (Z)-5-oxohex-2-enedioic acid. The enzyme was first isolated from Azotobacter vinelandii.
This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O<sub>2</sub> as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O<sub>2</sub>. The systematic name of this enzyme class is 1,2,3-trihydroxybenzene:oxygen 1,2-oxidoreductase (decyclizing). This enzyme is also called pyrogallol 1,2-dioxygenase.