Pyrimidine-deoxynucleoside 1'-dioxygenase from Rhodotorula glutinis () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are deoxyuridine, 2-oxoglutaric acid, and oxygen. Its products are 2-deoxyribonolactone, uracil, succinic acid, and carbon dioxide.
This enzyme is an alpha-ketoglutarate-dependent hydroxylase with systematic name 2'-deoxyuridine,2-oxoglutarate:oxygen oxidoreductase (1'-hydroxylating). It is also called deoxyuridine-uridine 1'-dioxygenase. It has 2 cofactors: iron, and ascorbic acid.
Pyrimidine-deoxynucleoside 2'-dioxygenase is a related enzyme which converts deoxyuridine to uridine instead of cleaving the uracil portion.