In enzymology, pyridoxine 4-oxidase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are pyridoxine and oxygen. Its products are pyridoxal and hydrogen peroxide.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is pyridoxine:oxygen 4-oxidoreductase. Other names in common use include pyridoxin 4-oxidase, and pyridoxol 4-oxidase. This enzyme participates in vitamin B<sub>6</sub> metabolism. It employs one cofactor, FAD.