The enzyme pyridoxal phosphatase (EC 3.1.3.74) catalyzes the reaction
It also acts on pyridoxine phosphate (PNP), pyridoxamine phosphate (PMP), 4-pyridoxic acid phosphate and 4-deoxypyridoxine phosphate to remove their phosphate groups. Two human genes produce proteins that act as this kind of enzyme: PDXP and PHOSPHO2.
This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. The systematic name is pyridoxal-5â²-phosphate phosphohydrolase. Other names in common use include vitamine B<sub>6</sub> (pyridoxine) phosphatase, PLP phosphatase, vitamin B<sub>6</sub>-phosphate phosphatase, and PNP phosphatase. This enzyme participates in vitamin B<sub>6</sub> metabolism.
As of late 2007, 6 structures have been solved for this class of enzymes, with PDB accession codes , , , , , and .