In enzymology, a progesterone monooxygenase () is an enzyme that catalyzes the chemical reaction
The 3 substrates of this enzyme are progesterone, AH<sub>2</sub>, and O<sub>2</sub>, whereas its 3 products are testosterone acetate, A, and H<sub>2</sub>O.
This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O<sub>2</sub> as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O miscellaneous. The systematic name of this enzyme class is . This enzyme is also called progesterone hydroxylase.