In enzymology, phenylglyoxylate dehydrogenase (acylating; ) is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are phenylglyoxylic aicd, coenzyme A, and oxidised nicotinamide adenine dinucleotide (NAD<sup>+</sup>). Its products are benzoyl-CoA, carbon dioxide, reduced NADH, and a proton.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the aldehyde or oxo group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is phenylglyoxylate:NAD+ oxidoreductase. It has 3 cofactors: FAD, Thiamin diphosphate, and Iron-sulfur.