In enzymology, a phenylacetyl-CoA dehydrogenase () is an enzyme that catalyzes the chemical reaction
The 3 substrates of this enzyme are phenylacetyl-CoA, H<sub>2</sub>O, and quinone, whereas its two products are phenylglyoxylyl-CoA and quinol.
This enzyme belongs to the family of oxidoreductases, specifically those acting on CH or CH<sub>2</sub> groups with a quinone or similar compound as acceptor. The systematic name of this enzyme class is phenylacetyl-CoA:quinone oxidoreductase. This enzyme is also called phenylacetyl-CoA:acceptor oxidoreductase.