Phenol 2-monooxygenase () is an enzyme that catalyzes the chemical reaction
The four substrates of this enzyme are phenol, reduced nicotinamide adenine dinucleotide phosphate (NADPH), oxygen and a proton. Its products are catechol, oxidised NADP<sup>+</sup>, and water.
The enzyme is a flavin-containing monooxygenase that uses molecular oxygen as oxidant and incorporates one of its atoms into the starting material. The systematic name of this enzyme class is phenol,NADPH:oxygen oxidoreductase (2-hydroxylating). Other names in common use include phenol hydroxylase, and phenol o-hydroxylase. It uses flavin adenine dinucleotide as a cofactor. The enzyme from Trichosporon cutaneum can hydroxylate a range of phenols.
As of late 2007, 3 structures have been solved for this class of enzymes, with PDB accession codes , , and .