In enzymology, a pentanamidase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are pentanamide and H<sub>2</sub>O, whereas its two products are valerate and NH<sub>3</sub>.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is pentanamide amidohydrolase. This enzyme is also called valeramidase.