In enzymology, a pantothenase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are (R)-pantothenate and H<sub>2</sub>O, whereas its two products are (R)-pantoate and beta-alanine.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is (R)-pantothenate amidohydrolase. Other names in common use include pantothenate hydrolase, and pantothenate amidohydrolase. This enzyme participates in pantothenate and coa biosynthesis.