In enzymology, a nucleoside phosphotransferase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are nucleotide and 2'-deoxynucleoside, whereas its two products are nucleoside and 2'-deoxynucleoside 5'-phosphate.
This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The systematic name of this enzyme class is nucleotide:nucleoside 5'-phosphotransferase. Other names in common use include nonspecific nucleoside phosphotransferase, and nucleotide:3'-deoxynucleoside 5'-phosphotransferase.