Nitrilotriacetate monooxygenase () is an enzyme with systematic name nitrilotriacetate,FMNH<sub>2</sub>:oxygen oxidoreductase (glyoxylate-forming). This enzyme catalyses the following chemical reaction
The three substrates of this enzyme are nitrilotriacetic acid, reduced flavin adenine dinucleotide (FADH<sub>2</sub>), and oxygen. It products are iminodiacetic acid, FAD, glyoxylic acid, and water. It requires Mg<sup>2+</sup>.