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Monoterpene epsilon-lactone hydrolase

The enzyme monoterpene ε-lactone hydrolase (EC 3.1.1.83, MLH; systematic name isoprop(en)ylmethyloxepan-2-one lactonohydrolase catalyses the reaction

(1) isoprop(en)ylmethyloxepan-2-one + H<sub>2</sub>O 6-hydroxyisoprop(en)ylmethylhexanoate (general reaction)
(2) 4-isopropenyl-7-methyloxepan-2-one + H<sub>2</sub>O 6-hydroxy-3-isopropenylheptanoate
(3) 7-isopropyl-4-methyloxepan-2-one + H<sub>2</sub>O 6-hydroxy-3,7-dimethyloctanoate

The enzyme catalyses the ring opening of ε-lactones in Gram-positive bacterium Rhodococcus erythropolis DCL14.

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