Methylglutamate dehydrogenase () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are N-methyl-L-glutamic acid, an electron acceptor, and water. Its products are L-glutamic acid, the corresponding reduced acceptor, and formaldehyde.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with other acceptors. The systematic name of this enzyme class is N-methyl-L-glutamate:acceptor oxidoreductase (demethylating). Other names in common use include N-methylglutamate dehydrogenase, and N-methyl-L-glutamate:(acceptor) oxidoreductase (demethylating). This enzyme participates in methane metabolism.