Methyl acetate hydrolase, known also as methylacetate acetohydrolase is a hydrolase enzyme that utilizes to carry out the hydrolysis of methyl acetate (CH<sub>3</sub>COOCH<sub>3</sub>), a weakly lipophillic and polar compound used often as a solvent. In the species Gordonia sp. (strain TY-5), the enzyme is encoded by gene acMB. The enzyme catalyzes the following reaction,
The enzyme is involved in two major pathways, the butanoate metabolism pathway and the catabolic degradation pathway of propanol.