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Methanogen homoaconitase

Methanogen homoaconitase (, methanogen HACN) is an enzyme with systematic name (R)-2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate-forming). This enzyme catalyses the following chemical reaction

(R)-2-hydroxybutane-1,2,4-tricarboxylate (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate (overall reaction)
: (1a) (R)-2-hydroxybutane-1,2,4-tricarboxylate (Z)-but-1-ene-1,2,4-tricarboxylate + H<sub>2</sub>O
: (1b) (Z)-but-1-ene-1,2,4-tricarboxylate + H<sub>2</sub>O (1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate

This enzyme catalyses several reactions in the pathway of coenzyme-B biosynthesis in methanogenic archaea.

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