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Lacto-N-biosidase

Lacto-N-biosidase () is an enzyme with systematic name oligosaccharide lacto-N-biosylhydrolase. This enzyme catalyses the following chemical reaction

(1) beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->4)-D-Glc + H<sub>2</sub>O beta-D-Gal-(1->3)-D-GlcNAc + beta-D-Gal-(1->4)-D-Glc
(2) lacto-N-tetraose + H<sub>2</sub>O lacto-N-biose + lactose

The enzyme from Streptomyces specifically hydrolyses the terminal lacto-N-biosyl residue (beta-D-Gal-(1->3)-D-GlcNAc) from the non-reducing end of oligosaccharides with the structure beta-D-Gal-(1->3)-beta-D-GlcNAc-(1->3)-beta-D-Gal-(1->R).

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