In enzymology, an isopenicillin N N-acyltransferase () is an enzyme that catalyzes the chemical reaction
The 3 substrates of this enzyme are phenylacetyl-CoA, isopenicillin N, and H<sub>2</sub>O, whereas its 3 products are CoA, penicillin G, and L-2-aminohexanedioate.
This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acyl-CoA:isopenicillin N N-acyltransferase. Other names in common use include acyl-coenzyme A:isopenicillin N acyltransferase, and isopenicillin N:acyl-CoA: acyltransferase. This enzyme participates in hydrophobic penicillins biosynthesis.