In enzymology, a homoserine O-acetyltransferase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are acetyl-CoA and L-homoserine, whereas its two products are CoA and O-acetyl-L-homoserine.
This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:L-homoserine O-acetyltransferase. Other names in common use include homoserine acetyltransferase, homoserine transacetylase, homoserine-O-transacetylase, and L-homoserine O-acetyltransferase. This enzyme participates in methionine metabolism and sulfur metabolism.
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .