In enzymology, a glycerol dehydratase () catalyzes the chemical reaction:
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is glycerol hydro-lyase (3-hydroxypropanol-forming). Other names in common use include glycerol dehydrase, and glycerol hydro-lyase. This enzyme participates in glycerolipid metabolism. It employs one cofactor, cobalamin.
As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes and .