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Glucosaminate ammonia-lyase

The enzyme Glucosaminate ammonia-lyase (EC 4.3.1.9) catalyzes the chemical reaction

2-amino-2-deoxy-<small>D</small>-gluconate = 2-dehydro-3-deoxy-<small>D</small>-gluconate + NH<sub>3</sub> (overall reaction)
:(1a) 2-amino-2-deoxy-<small>D</small>gluconate = (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate + H<sub>2</sub>O
:(1b) (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate = (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate (spontaneous)
:(1c) (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate + H<sub>2</sub>O = 2-dehydro-3-deoxy<small>D</small>-gluconate + NH<sub>3</sub> (spontaneous)

This enzyme belongs to the family of lyases, specifically ammonia lyases, which cleave carbon-nitrogen bonds. The systematic name of this enzyme class is 2-amino-2-deoxy-<small>D</small>-gluconate ammonia-lyase (isomerizing; 2-dehydro-3-deoxy-<small>D</small>-gluconate-forming). Other names in common use include glucosaminic dehydrase, <small>D</small>-glucosaminate dehydratase, <small>D</small>-glucosaminic acid dehydrase, aminodeoxygluconate dehydratase, 2-amino-2-deoxy-<small>D</small>-gluconate hydro-lyase (deaminating), aminodeoxygluconate ammonia-lyase, 2-amino-2-deoxy-<small>D</small>-gluconate ammonia-lyase, and <small>D</small>-glucosaminate ammonia-lyase. This enzyme participates in the pentose phosphate pathway. It employs one cofactor, pyridoxal phosphate.

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