The enzyme gluconate dehydratase () catalyzes the chemical reaction
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is <small>D</small>-gluconate hydro-lyase (2-dehydro-3-deoxy-D-gluconate-forming). Other names in common use include <small>D</small>-gluconate dehydratase, and <small>D</small>-gluconate hydro-lyase. This enzyme participates in the pentose phosphate pathway.
As of late 2007, only one structure has been solved for this class of enzymes, with the PDB accession code .