Flavonoid 3',5'-hydroxylase ( was wrongly classified as in the past) is an enzyme with systematic name flavanone,NADPH:oxygen oxidoreductase. This enzyme catalyses the following chemical reaction
flavanone + 2 NADPH + 2 H<sup>+</sup> + 2 O<sub>2</sub> 3',5'-dihydroxyflavanone + 2 NADP<sup>+</sup> + 2 H<sub>2</sub>O (overall reaction)
(1a) flavanone + NADPH + H<sup>+</sup> + O<sub>2</sub> 3'-hydroxyflavanone + NADP<sup>+</sup> + H<sub>2</sub>O
(1b) 3'-hydroxyflavanone + NADPH + H<sup>+</sup> + O<sub>2</sub> 3',5'-dihydroxyflavanone + NADP<sup>+</sup> + H<sub>2</sub>O
Flavonoid 3',5'-hydroxylase is a heme-thiolate protein (P-450).
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