Exodeoxyribonuclease III (EC 3.1.11.2, Escherichia coli exonuclease III, E. coli exonuclease III, endoribonuclease III) is an enzyme that catalyses the following reaction
Exonucleolytic cleavage in the 3â²- to 5â²-direction to yield nucleoside 5â²-phosphates
This enzyme has a preference for double-stranded DNA.