In enzymology, ephedrine dehydrogenase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are ephedrine and oxidised nicotinamide adenine dinucleotide (NAD<sup>+</sup>). This forms an imine intermediate which spontaneously hydrolyses to give (-)-phenylacetylcarbinol and methylamine. The enzyme was isolated from a strain of the soil bacterium Pseudomonas putida which uses ephedrine as its sole source of carbon.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (-)-ephedrine:NAD+ 2-oxidoreductase.