Eicosanolide is an organic compound with the chemical formula . It is a cyclic ester or lactone, more specifically a macrolide.
Eicosanolide is used by several species of bees (such as some of genera Colletes, Lasioglossum, Halictus) and butterflies (such as some of genus Heliconius) as a pheromone. The Dufour's gland of bees in the Halictinae subfamily, contains eicosanolide along with other macrocyclic lactones, which could be used for a range of different applications like nest building, larval food and chemical communication.