The enzyme dimethylmaleate hydratase () catalyzes the chemical reaction
This enzyme belongs to the family of lyases, specifically the hydro-lyases, which cleave carbon-oxygen bonds. The systematic name of this enzyme class is (2R,3S)-2,3-dimethylmalate hydro-lyase (dimethylmaleate-forming). This enzyme is also called (2R,3S)-2,3-dimethylmalate hydro-lyase. This enzyme participates in c5-branched dibasic acid metabolism. It employs one cofactor, iron.