Deacetoxycephalosporin-C hydroxylase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are deacetoxycephalosporin C and oxygen. Its product is deacetylcephalosporin C.
This enzyme is an oxidoreductase with systematic name deacetoxycephalosporin-C,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating). Other names in common use include deacetylcephalosporin C synthase, 3'-methylcephem hydroxylase, DACS, DAOC hydroxylase, and deacetoxycephalosporin C hydroxylase. This non-heme iron protein generates a ferryl group at its active site; Fe(IV)=O is the species that transfers its oxygen to the substrate.
The mechanism used by these 2-oxoglutarate-dependent oxygenases requires 2-oxoglutaric acid to activate the iron oxygen complex, and this gives succinic acid and carbon dioxide when the second atom of the molecular oxygen is removed.
This enzyme is involved in the biosynthesis of cephalosporin C in Acremonium chrysogenum, which is used for the industrial production of that antibiotic.