In enzymology, cyclohexylamine oxidase () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are cyclohexylamine, water, and oxygen. Its products are cyclohexanone, hydrogen peroxide, and ammonia.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH<sub>2</sub> group of donors with oxygen as acceptor. The systematic name of this enzyme class is cyclohexylamine:oxygen oxidoreductase (deaminating). This enzyme participates in caprolactam degradation. It employs one cofactor, FAD.