In enzymology, cyclohexanone dehydrogenase () is an enzyme that catalyzes a redox chemical reaction of the form
The two substrates of this enzyme are cyclohexanone and a electron acceptor. Its products are cyclohexenone and reduced acceptor.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-CH group of donor with other acceptors. The systematic name of this enzyme class is cyclohexanone:acceptor 2-oxidoreductase. This enzyme is also called cyclohexanone:(acceptor) 2-oxidoreductase.